Hi all,
I was wondering whether there are different reagents used for a single end versus a paired end sequencing run on HiSeq?
The following presentation (http://skatebase.org/sites/skatebase...lluminaNGS.pdf) nicely illustrates the difference in oligos when bound to a flow cell for single-end versus paired end sequencing (page 41-42). However, it was told to me that you can use the same reagents for both sequencing protocols but according to this ppt, either a chemical (periodate) or an enzyme is used for linearization of the P5 oligo before Read 1 primer binding suggesting different required reagents. Can anyone share some ideas about this?
Thanks a lot!
I was wondering whether there are different reagents used for a single end versus a paired end sequencing run on HiSeq?
The following presentation (http://skatebase.org/sites/skatebase...lluminaNGS.pdf) nicely illustrates the difference in oligos when bound to a flow cell for single-end versus paired end sequencing (page 41-42). However, it was told to me that you can use the same reagents for both sequencing protocols but according to this ppt, either a chemical (periodate) or an enzyme is used for linearization of the P5 oligo before Read 1 primer binding suggesting different required reagents. Can anyone share some ideas about this?
Thanks a lot!
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