Does anyone have any experience performing target enrichment from pooled libraries ? We're very interested in performing SureSelect (Agilent) in-solution capture (or array-based capture) with pooled libraries (SOLiD or Illumina). We'd like to pool 16 barcoded libraries and perform a single enrichment. Has anyone tried this ?
We would expect there to be a limit to the number of individuals that can be pooled, while still retaining sufficient sequence complexity after hybridization across all targets.
We would expect there to be a limit to the number of individuals that can be pooled, while still retaining sufficient sequence complexity after hybridization across all targets.
Comment