Good day everyone,
I've nearly 2GB genomic data of a newly assembled genome in nearly 1 million scaffolds.
Also I've a 100MB 454 reads (average read length ~350nt).
I would like to align/map all the 454 reads against the scaffolds and view it in an alignment viewer. The objective is to find and extract those scaffold regions which have highest number of 454 reads aligned to it.
Any particular tool/code/pipeline/suggestions will be greatly appreciated.
Regards.
I've nearly 2GB genomic data of a newly assembled genome in nearly 1 million scaffolds.
Also I've a 100MB 454 reads (average read length ~350nt).
I would like to align/map all the 454 reads against the scaffolds and view it in an alignment viewer. The objective is to find and extract those scaffold regions which have highest number of 454 reads aligned to it.
Any particular tool/code/pipeline/suggestions will be greatly appreciated.
Regards.
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