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Old 11-29-2012, 01:11 AM   #1
Afazja
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Location: PL

Join Date: Nov 2012
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Default microrna and small rna library prep for ion Torrent v. miseq

We are interested in deep sequencing of the small/microRNA and wandering on decision which platform to select - Ion Torrent or MiSeq. Can anyone help us with a comment on a library preparation for small RNA sequencing for both platforms. What is better/cheaper/quicker/more "sample efficient" (less input material/total RNA required)? My understanding is that Ion Torrent protocol can avoid gel extraction is there really a great advantage over MiSeq protocol? Thanks in advance for your tips.

Marcin
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