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Old 04-09-2013, 05:08 AM   #1
mikev
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Location: the netherlands

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Default RRBS acceptable coverage

Hi all,

I am new in RRBS. I want to use the gel-free protocol descripted in the article of Boyle et al. Genome biology 2012, 13. I want to use RRBS for a population based studie. What is an acceptable coverage per CpG for this type of studie?
I ask this to determine the maximal acceptable number of samples per lane. I want to run on a hiseq 2000.
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coverage depth, epigenetics, methylation, multiplexing, rrbs

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