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Old 11-17-2009, 09:52 AM   #1
Location: Padova ITALY

Join Date: Jul 2009
Posts: 32
Question Enrichment with AGILENT's Sureselect target enrichment system

I was wondering if anybody has ever tried to enrich a dna sample for target re-sequencing with a 454 using Agilent's liquid enrichment system (now officially available just for Illumina and Solid).
We are trying alternative enrichment methods to target a 100kb-1Mb sequence for many samples.
Nimblegen looks like has copied Agilent's approach for the enrichment with its new SeqCap EZ exome but it seems to be really expensive expecially because I ve been told there's a minimum of ~50 reaction kit to buy.. and first of all the kit is only available for the exome enrichement and not for custom design.
We are trying with Combimatrix, but the cheap kit from agilent could be another good product to try.

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Old 11-18-2009, 02:14 AM   #2
Location: Belgium

Join Date: Nov 2008
Posts: 79

SureSelect followed by 454 sequencing will be possible end of Februari 2010 (the earliest)
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