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Old 01-28-2011, 09:05 AM   #1
athos
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Default Agilent Sure Select

I have a question about the Sure Select Multiplex kit - I notice that they use a different primer to that which is included in the Illumina multiplex sample prep kit. Does anyone know what the sequences of the Sure Select Indexing Pre-capture PCR primer and post-capture PCR primer are?
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Old 01-28-2011, 09:06 AM   #2
DMO
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Its proprietary, I have asked. If anyone has it, I would be very interested in knowing it as well.
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Old 01-28-2011, 01:47 PM   #3
upenn_ngs
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I believe the pre-capture PCR primer is equivalent to the Illumina Multiplexing PCR Primer 2.0

The post-capture PCR primer is simply the Illumina Multiplexing PCR Primer 1.0 .

I cannot confirm this, but it makes logical sense.
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