Hi all,
I'm preparing libraries from very small amounts of RNA, around 100-1000x less than the 1ug recommended by illumina. Generating a "library" seems easy but on analysis it turns out to be mostly adaptor. Has anyone experimented with adding less adaptor? Or any other ways of optimizing library preparation for small amounts of nucleic acids?
I get the impression this is a fairly common problem, I'll be on the phone to illumina in a bit and I'll pass along any gems they give me. In the meantime what do all you seqanswerers think?
WW
I'm preparing libraries from very small amounts of RNA, around 100-1000x less than the 1ug recommended by illumina. Generating a "library" seems easy but on analysis it turns out to be mostly adaptor. Has anyone experimented with adding less adaptor? Or any other ways of optimizing library preparation for small amounts of nucleic acids?
I get the impression this is a fairly common problem, I'll be on the phone to illumina in a bit and I'll pass along any gems they give me. In the meantime what do all you seqanswerers think?
WW
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