My lab is new to NGS, and we are looking to map the regulon of the TyrR transcription factor in our bacterium, E. cloacae. In general, we wish to identify TyrR-DNA interactions in response to the presence or absence of aromatic amino acids, which function as cofactors for TyrR binding. This would give use 12 samples in total, a no AAA control, +TRP, +PHE and +TYR all in triplicate.
We are thinking on using Illumina sequencing and multiplexing our samples so that they can all be run in a single lane. Is there a good comprehensive resource that details the entire procedure from start to end? I have a good hold on most of the steps involved, but I do not know enough yet to properly decide on how many reads, type of sequencing, etc. Thanks in advance.
We are thinking on using Illumina sequencing and multiplexing our samples so that they can all be run in a single lane. Is there a good comprehensive resource that details the entire procedure from start to end? I have a good hold on most of the steps involved, but I do not know enough yet to properly decide on how many reads, type of sequencing, etc. Thanks in advance.
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