Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • #1

    Restriction digest of RNA /DNA hybrid

    Has anyone used a restriction enzyme on a 1st strand cDNA synthesis product

    what would I expect if the SSpI RE is used on a viral cDNA 1st strand product if the site is available on a dsDNA sequence of this genome?
    Tags: None
  • #2
    Perhaps more luck getting an answer in the Sample Prep / Library Generation forum...

    Comment

    Previous template Next

    Latest Articles

    Collapse
    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM
    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM
    View All

    ad_right_rmr

    Collapse

    News

    Collapse
    Topics Statistics Last Post
    Cancer Metastasis: A Deep Dive into Cellular Plasticity by seqadmin
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    31 views
    0 likes
    		 Last Post seqadmin
    by seqadmin
    04-11-2024, 12:08 PM  
    Proteogenomic Profiles Offer New Clues in Prostate Cancer by seqadmin
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    32 views
    0 likes
    		 Last Post seqadmin
    by seqadmin
    04-10-2024, 10:19 PM  
    Novel Diagnostic Assay Enhances Ovarian Cancer Detection by seqadmin
    Started by seqadmin, 04-10-2024, 09:21 AM
    0 responses
    28 views
    0 likes
    		 Last Post seqadmin
    by seqadmin
    04-10-2024, 09:21 AM  
    Evolutionary Dynamics of Centromeres: A Comparative Genomic Analysis by seqadmin
    Started by seqadmin, 04-04-2024, 09:00 AM
    0 responses
    53 views
    0 likes
    		 Last Post seqadmin
    by seqadmin
    04-04-2024, 09:00 AM  
    View All
    Working...
    X