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Old 07-05-2011, 06:40 AM   #1
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Default PubMed: Metagenomic comparison of direct and indirect soil DNA extraction approaches.

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Metagenomic comparison of direct and indirect soil DNA extraction approaches.

J Microbiol Methods. 2011 Jun 25;

Authors: Delmont TO, Robe P, Clark I, Simonet P, Vogel TM

Full pyrosequencing runs of both direct-extracted (high yield, low DNA size) and indirect-extracted DNA (low yield, high DNA size) from the same prairie soil show that the sequence distribution of the majority of the metabolic functions and species detected were statistically similar. Although some microbial functions differed at the 95% confidence interval in bootstrap analyses, the overall functional diversity was the same.

PMID: 21723887 [PubMed - as supplied by publisher]



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