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Old 10-26-2008, 06:50 PM   #1
seqgirl123
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Location: U.S

Join Date: Oct 2008
Posts: 76
Question templiphi dna extraction

hello,
has anyone used templiphi dna to amplify dna without pcr? i do not see any posts about templiphi here.

i have used templiphi to amplify the dna of a pUC18 control they give in the kit and a bacterial liquid culture of my own. the sequencing came out quite well.

I would like to isolate the templiphi'd dna of the control and my bacterial culture. there is no protocol i can find that shows how to do this. i would like to place the isolated dna on a slide. the booklet gives an isolation procedure for BAC DNA, but not bacterial cultured dna.

please help with any advice.
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