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Thread | Thread Starter | Forum | Replies | Last Post |
Anybody own an EpMotion 5075 Liquid Handler? | jlove | Illumina/Solexa | 0 | 12-16-2011 12:20 PM |
PubMed: Performance of microarray and liquid based capture methods for target enrichm | Newsbot! | Literature Watch | 0 | 02-25-2011 11:20 AM |
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#1 |
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Location: Boston, MA Join Date: Apr 2011
Posts: 48
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We're looking into liquid handlers and we checked out the Eppendorf EpMotion 5075 recently. We're doing our due diligence - does anybody have any reviews or experience to share? We'd be looking for it to set up reactions and do bead purifications for Illumina TruSeq libary preps.
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#2 |
Junior Member
Location: USA Join Date: Dec 2011
Posts: 1
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[QUOTE=jlove;59829]We're looking into liquid handlers and we checked out the Eppendorf EpMotion 5075 recently. We're doing our due diligence - does anybody have any reviews or experience to share? We'd be looking for it to set up reactions and do bead purifications for Illumina TruSeq libary preps.[/QUOTE
The best thing you can do is push for them to bring an instrument in the lab and make them run some preps for you. They will do this for you. I would keep an eye on the purifications. There tends to be beads left behind. This can be a problem. If you don't mind manually getting rid of the beads between runs on the instrument, then you should be OK. It is key to make sure they prove the instrument works. They don't own any chemistry to tie to the instrument. This is always a huge risk. Who will you call when there is a failure? Illumina? Eppendorf? You may get ignored and get stuck with an instrument that doesn't work. |
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#3 |
Junior Member
Location: StL, Mo Join Date: Jan 2012
Posts: 2
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Hi,
We ran the EP exclusively for our Illumina Lib preps as well as qPCR set up. Some tweeks were needed regarding labware definitions and aspiration speeds/depths but once we worked out the kinks we fell in love. |
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#4 |
--Site Admin--
Location: SF Bay Area, CA, USA Join Date: Oct 2007
Posts: 1,352
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For canned protocols, it's pretty nice. For things requiring lots of flexibility, there are better instruments/interfaces.
I also recall it being pretty expensive....but their sales/tech support guys were very helpful throughout (pre- and post- sale). |
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#5 |
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Location: Boston, MA Join Date: Apr 2011
Posts: 48
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Hi firedancer - thanks for the feedback. I'd be interested to hear about any specific tweaks. (if you can easily recall any details).
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#6 | |
Junior Member
Location: StL, Mo Join Date: Jan 2012
Posts: 2
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We used it for multiple apps but mainly for Next gen lib preps and such. The main hurdle with the instrument was getting the depths just right to perform spri cleanups. Liquid was getting left in the wells or beads were getting pulled off the magnets. After asking the eppendorf crew to define the labware (magnet) and messing around with pipette heights and speeds we got this step to work pretty well. |
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#7 | |
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Location: TN Join Date: Oct 2011
Posts: 11
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One thing to consider is that there is no 96 well head, so you do full plate processing 8 wells at a time. And I find it rather slow, not exactly sure how it compares to other platforms in that department, however. |
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#8 |
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Location: Texas Join Date: Jul 2012
Posts: 26
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I know this thread is old, but curious if anyone has any updates on their experience with the 5075, in particular the 5075 TMX platform, or any competitor automation platforms (SciClone, etc) for NGS library prep?
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#9 |
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Location: Oregon Join Date: Apr 2008
Posts: 22
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Any protocols you are willing to share, firedancer and behavin? We've just installed a 5075 with the desire to set up KAPA qPCR validation plates in addition to library preps. I know we can develop them ourselves, but why not stand on the shoulders of giants?
Thanks in advance |
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#10 |
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Location: Oregon Join Date: Apr 2008
Posts: 22
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Hi Skamp. I would be very interested in your DNA extraction protocols (and thanks for the message about the tips!). I have not heard anything else about protocols in place for library preps (e.g. TruSeq).
Thanks again |
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#11 |
Junior Member
Location: San Diego Join Date: Sep 2013
Posts: 4
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We have a 5075 at our core that we are utilizing for NG Library preps. There is a bit of a learning curve and the writing protocols/ troubleshooting is a bit cumbersome, but the Eppendorf sales and tech team are amazing and very helpful. They are always ready and willing to come out to help troubleshoot. We have almost worked out all the kinks for automating TruSeq RNA lib preps and even though there is still quiet a lot of user intervention, I think that the robot was a good buy.
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#12 | |
Junior Member
Location: UK Join Date: Oct 2011
Posts: 4
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how do you address the issue with beads carry over? does it make sense to try different magnetic stands or are they all the same? |
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#13 |
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Location: Texas Join Date: Jul 2012
Posts: 26
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Can't help you with the first part of the question, but in terms of bead carryover I've found it can often be addressed by slowing down the speed of the pipetting either in automated or manual applications. Also, I think the stand matters - I've tried both the "O-ring magnet" style and the "post" magnet style (24? posts arrayed such that they're on the side wall of the individual tubes in the 96 well plate) and I find the post magnet style to give superior results in our hands.
Best of luck docbio |
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#14 |
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Location: Corpus Christi, TX Join Date: May 2012
Posts: 10
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We have an epMotion 5075, and it is awful. You have very little control over the machine, the software is sophmoric. It wastes tips, can't be customized, ependorf won't let you make your own labware definitions so you're hostage to their 1 employee that does it, you have no access to the coding language to fix their poor software routines, customer support sucks. Don't buy
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#15 |
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Location: Corpus Christi, TX Join Date: May 2012
Posts: 10
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On a related note, now that I'm stuck with this thing, I want to hack the machine so we can make it do what we want. Anybody ever hacked an epmotion? Or better yet, does anybody know what company eppendorf bought this robot from? Typcally, the older robots came with programming guides etc
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#16 | |
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Location: TN Join Date: Aug 2014
Posts: 1
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#17 |
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Location: Missoula, MT Join Date: Jan 2014
Posts: 19
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Are there any updates here regarding good or bad reviews of the epMotion 5075 TMX? Or other models?
Thanks! |
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#18 |
Junior Member
Location: Ann Arbor, MI Join Date: Aug 2013
Posts: 9
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I like the epMotion 5075. I think its a great little robot, especially if you are new to programming these types of devices. I like that it checks to make sure the proper labware is being used and has a lot of fail safes for people who use the device infrequently.
That being said, I do like that the BioMex allows you to define your own labware and you can certainly customize it more then the epMotion, but you have to have the training and time to do that. It does not 'check' to ensure the labware is correct or even present, so I found it to be much more time consuming to learn to program and use. Last edited by acockburn; 12-10-2014 at 06:13 AM. |
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#19 |
Junior Member
Location: germany Join Date: Sep 2015
Posts: 1
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Hi,
we have a epMotion 5070 PC and an older 5075 control panel. True, they are terribly unflexible. With some trial and error one can define own labware but it's more or less undocumented. We have our own 3D printed plates and had to go through this... But I still could not find a way to define my own methods, or better, to control the machine with matlab instead of epBlue. E.g. i need the machine to pipette while moving. Does anyone have experience on that? Best Tim |
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