SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
Ampure XP buffer cli Sample Prep / Library Generation 8 12-16-2014 09:26 PM
Pippin Prep vs. gel elution for RNA-Seq shurjo Sample Prep / Library Generation 2 07-04-2013 03:30 PM
Alternative DNA elution for ChIPseq crocky Sample Prep / Library Generation 3 10-15-2012 01:15 AM
elution from streptaviden beads shawpa Sample Prep / Library Generation 1 07-09-2012 12:17 AM
Elution volume after A-tailing? greigite Illumina/Solexa 2 11-01-2009 01:45 PM

Reply
 
Thread Tools
Old 02-17-2013, 04:41 AM   #1
stasi
Junior Member
 
Location: Israel

Join Date: Feb 2013
Posts: 4
Default elution buffer Question

hello
I work with illumina (TruSeq) kits for RNA and DNA sample preparation.
Unfortunately, I spilled the elution buffer (for RNA prep). I try to avoid ordering a complete kit. Maybe someone knows what buffer can replace it or maybe know another kit (cheaper) containing the elution buffer.
Thanks
stasi is offline   Reply With Quote
Old 02-17-2013, 05:34 AM   #2
biznatch
Senior Member
 
Location: Canada

Join Date: Nov 2010
Posts: 126
Default

You might be able to order just that elution buffer from illumina. We use Qiagen kits for lots of things and have ordered individual buffers on several occasions.
biznatch is offline   Reply With Quote
Old 02-18-2013, 05:13 AM   #3
pmiguel
Senior Member
 
Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,313
Default

In the US Illumina will generally replace a component if you accidentally spill it. Call Tech support and/or your sales rep for help.

--
Phillip
pmiguel is offline   Reply With Quote
Old 02-22-2013, 03:38 PM   #4
kcchan
Senior Member
 
Location: USA

Join Date: Jul 2012
Posts: 182
Default

As pmiguel said, Illumina is generally very good about replacing reagents. If you're in a bind you can also just use Tris-HCl or even nuclease free water for the bead elution.
kcchan is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 06:04 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO