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Old 12-14-2015, 12:55 PM   #1
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Location: Edinburgh, UK

Join Date: Dec 2015
Posts: 1
Default RQ1 DNase I digestion of Total RNA

I am having problems with the quality of RNA after RQ1 DNase I treatment. After DNase treatment, I use phen/Chlor for RNA purification. I use ethanol for final precipitation. If you look at my bioanlyser trace before and after DNase digesition, there is a large pick after ribosomal RNA. I do not know what this is. If I heat the RNA 6 minutes at 65, I do not see this extra pick but the quality of the RNA drops a lot. I am worried that this pick can affect the Ribo-Zero step and library Prep.

Does anybody had this problem?

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File Type: png Befor DNase I digestion.png (6.9 KB, 5 views)
File Type: jpg After DNase I digestion.jpg (34.7 KB, 5 views)
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