Dear all,
As captioned, this sequencing included 4 barcoded libraries prepared by the following protocol, and barcode lbc01, lbc02, lbc03 and lbc04 were chosen, respectively. The library size is around 7.5kb (the longest subread is 5259 bp), and the SMRT Link we use is Version 4.
Refer to attached, demultiplexing the data with full set of 96 barcodes, the problem is that most of the sequencing reads are sorted as other 92 no-existing barcodes, but the 4 barcodes we used!!!!
I think the size as 5,259 bp is too long for PacBio demultiplex.
Anyone has experience on sequencing barcoded libraries?? suggestions?
Thanks,
Wei
As captioned, this sequencing included 4 barcoded libraries prepared by the following protocol, and barcode lbc01, lbc02, lbc03 and lbc04 were chosen, respectively. The library size is around 7.5kb (the longest subread is 5259 bp), and the SMRT Link we use is Version 4.
Refer to attached, demultiplexing the data with full set of 96 barcodes, the problem is that most of the sequencing reads are sorted as other 92 no-existing barcodes, but the 4 barcodes we used!!!!
I think the size as 5,259 bp is too long for PacBio demultiplex.
Anyone has experience on sequencing barcoded libraries?? suggestions?
Thanks,
Wei
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