Tweet
I got too few significantly differently expressed genes between two samples, was something wrong. and how did cuffdiff call a gene significantly differently expressed
-
-
1) How do you know its too few?
2) "two samples" do you mean multiple biological replicates of two conditions or only two samples? It makes a big difference. -
1）I got just 68 genes significantly differently expressed
2) one is cancer cell line,the other is normal tissueComment
-
1）I got just 68 genes significantly differently expressedOriginally posted by chadn737 View Post
2) one is cancer cell line,the other is normal tissueComment
-
1) Why is 68 too few?
2) So you have only two samples, no replicates?Comment
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment