Hello,
I would be using this kit for single cell RNA in a 96 well plate. I will be preparing the reactions in a plate. I would like to know if anyone has spiked in ERCC or Array Control RNA Spikes into the lysis mastermix or any internal RNA controls into the reaction, and how did you do this? Did you spike it into the lysis mastermix, and if yes, at what volume of this versus the other lysis reagents? Preferably i will like to use ERCC spike ins, how do I spike them into the reaction setup?
My other question is I would like to have the RT step barcoded per single cell. Where can I get 96 barcodes and how to add them into the RT step of this workflow?
I would be using this kit for single cell RNA in a 96 well plate. I will be preparing the reactions in a plate. I would like to know if anyone has spiked in ERCC or Array Control RNA Spikes into the lysis mastermix or any internal RNA controls into the reaction, and how did you do this? Did you spike it into the lysis mastermix, and if yes, at what volume of this versus the other lysis reagents? Preferably i will like to use ERCC spike ins, how do I spike them into the reaction setup?
My other question is I would like to have the RT step barcoded per single cell. Where can I get 96 barcodes and how to add them into the RT step of this workflow?
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