hello,
i'm new user of illumina miseq technology and i'm finding some difficulties understanding some basics.
if i select in the machine that i want to get fragments of 250 bp why do i still get fragments from 35 bp to 250 bp in my output? ( i don't know how the fragmentation step is done, if the transposon generate only the fragments of 250 bP or different sizes)
and does the adapters used are automaticaly removed from the fragments in the output files?
could someone help me to understand
i'm new user of illumina miseq technology and i'm finding some difficulties understanding some basics.
if i select in the machine that i want to get fragments of 250 bp why do i still get fragments from 35 bp to 250 bp in my output? ( i don't know how the fragmentation step is done, if the transposon generate only the fragments of 250 bP or different sizes)
and does the adapters used are automaticaly removed from the fragments in the output files?
could someone help me to understand
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