Resequencing Old Libraries

[slevine]

Hello,

Not sure if there is an existing discussion on this, but I don't have great keywords to use..

We were looking to resequence some older libraries as part of a larger pool of new samples (MiSeq PE150). Post run, we're not observing any reads from the older samples. The older libraries were stored at -20C for about 1.5y. We reconfirmed concentration by qPCR before pooling and reconfirmed the BioA traces after the run and saw no differences between when the libraries were created and sequenced the first time versus now. Looking for any help in understanding what might have gone wrong or anyone with experience resequencing libraries over a year old.

Thanks!

[HESmith]

How were the libraries constructed (i.e., which kit/version was used)? Some older libraries are not compatible with the newer flow cell/primer sets.