Anyone seen anything like the sharp peaks at 250 bp in the bioanalyzer traces of the libraries below? Overamplified? Bubble product?
These are from rRNA depleted (Ribo zero) total RNA from E. coli. Prepared with a TruSeq Stranded Total RNA kit with the default fragmentation conditions.
These are from rRNA depleted (Ribo zero) total RNA from E. coli. Prepared with a TruSeq Stranded Total RNA kit with the default fragmentation conditions.
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