Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
QC of ATAC-seq libraries on MiSeq Nano OlgaMikh Illumina/Solexa 2 04-24-2019 06:11 AM
MiSeq Micro/Nano Kits v2 for 16S? jluk Metagenomics 2 08-03-2016 05:40 AM
MiSeq v3 kit for high diversity vs. low diversity libraries phyllosphere Illumina/Solexa 4 12-23-2015 12:29 PM
How to check if reads are properly paired in mate-pair data? genepool_bee Bioinformatics 2 02-22-2011 01:07 AM

Thread Tools
Old 01-17-2020, 06:48 PM   #1
Junior Member
Location: NYC

Join Date: Oct 2019
Posts: 3
Default MiSeq Nano 1M reads for whole-gen diversity check

I recently submitted the GecKO library (~120K guides) for a MiSeq Nano run to check diversity (and mostly to check my custom seq primer etc. was working). It performed 1.3M reads, so about 10x the library size. I realize this is not nearly enough, since it's recommended to do 100x the library size. Even with 1/10th of the recommended depth, I had about 2.5% unread guides (rec is <0.5%). Do you think I can go ahead with this library? I really don't want to spend more money to do a 12M+ run. Thoughts?
chilipep is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 06:01 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO