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Old 01-05-2013, 01:45 PM   #1
Daytwa
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Location: Detroit

Join Date: Dec 2010
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Default LinDA Troubleshooting

Has anyone had consistent success using Shankaranarayanan et al.'s Single-tube linear DNA amplification (LinDA) for robust ChIP-seq protocol?

We have yet to yield more than 10 ng of RNA. This yields about 2 ng of DNA in our hands.

We are using nuclease free water and tubes. The IVT kit and buffers recommended by the authors. We do notice that when setting up the IVT reaction our samples become a light tan color (oxidation?) and after a 16 hour incubation a brown precipitate has formed.

Any thoughts or suggestions would be great. Thanks!
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