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Hello everyone! I am using QIAEX II Gel Extraction Kit to purify PCR products (300 bp) from agarose gel. The final elution is in 20 ul of H2O. I use 5 ul of the eluate in a BigDye Terminator sequencing reaction and then I purify my product using a Membrane-based filter plate. The sequence I get is unfortunately very bad quality , with some spikes and a lot of noise. Do you think I should change seq purifiction method?
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM