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Old 03-16-2014, 11:48 PM   #1
Nilaksha
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Location: Sri Lanka

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Default Human Exome Sequencing using Ion torrent PGM

As the web page of Life technologies said, Ion torrent PGM is for small genomes and targeted gene expressions while the Ion proton is for human genomes and exomes. Yet, I came across that ion torrent PGM can be used in exome sequencing using 318 kits in some references but the information given are rather scanty.
To my knowledge there is only one NGS sequencing machine in my country and it's an ion torrent PGM.
Any support or information you know about exome sequencing via ion torrent PGM is much appreciated.
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Old 03-17-2014, 08:30 AM   #2
AllSeq
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Nilaksha,

There's nothing inherently wrong about using the 318 chip for exome sequencing, it's just not the most efficient and will be more expensive than using a PI chip (or a MiSeq). People generally want ~5Gb of coverage for a human genome (100X coverage of 50Mb of sequence). The 318 chip tops out around 2Gb, so you're either stuck with lower coverage or you'll need to run 2-3 chips (adding to the expense). At AllSeq we've compiled the Ion Torrent specs for easy comparison.

If you're willing to use a provider outside of Sri Lanka, I'd encourage you to check out our Sequencing Marketplace. We'll help match you with a provider with any technology you'd like.

Sincerely,
The AllSeq Support Team
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Old 03-17-2014, 07:37 PM   #3
Nilaksha
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Thank you very much for the valuable information. I checked AllSeq, and it is very informative.
I will go through the site further and ask you if I get any doubts.

Thanking you,
Nilaksha.
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