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Old 04-15-2014, 10:41 PM   #1
Location: Barcelona

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Default Reads mapped to another chromosome in paired-end data of RNA-seq

Hello guys,

I am doing a project in RNA-seq data with some datasets that are paired-end data. After alignment with TopHat2, I have around 1-5% of reads whose mate is mapped to another chromosome. In order to call Cufflinks for annotation of transcripts, would you recommend me to remove those reads mapped to another chromosome (or call again TopHat2 with the option --no-discordant)? Is it a key step?

Thanks in advanced.
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Old 04-21-2014, 10:00 AM   #2
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If the RNA is from cell lines this would make sense since they have numerous translocations and duplications.
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