I'm in the process of testing some various sequencing techniques with reads of various lengths. I have one data set I would particularly like to use because of its read length, but it is a paired end read; I am only testing on non-paired end reads. Would it be valid for me to remove the /1 or /2 from the ends of the id sequence (FASTQ Illumina formatting) and then run all the lanes through? Or should I only run half of every lane through (for example, all the 1 halves of the pairs? Or is even trying to do this misrepresenting the data?
Thanks.
Thanks.
Comment