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Old 02-28-2016, 01:25 AM   #1
Kilaza Samson
Junior Member
Location: Tanzania

Join Date: Aug 2012
Posts: 1
Question Problem with uploading BIOM data file from Mothur into MEGAN 5.11.3

Hallow members! I am pleased to join this forum and hope to learn from members.
I am experiencing a problem with upload BIOM data file into MEGAN 5.11.3. The V4 - 16S rRNA gene sequencing was done using Illumina MiSeq platform, and the QC and statistical analyses were done using Mothur package. When I am trying to upload the BIOM file into MEGAN 5.11.3 for further analysis I am getting this error
"Execute failed: cannot be cast to”
This error has happened following an update of the MEGAN 5.10 to MEGAN 5.11.3. In earlier version, there was no problem. I have tried re installing the package and following steps given in manual (Including installation of recent java runtime environment (jre) v 1.7, still I cannot upload I have also tried to a different computer, still could not make it. I am not knowledgeable of the command line approach would have tried. Can someone give me a hand on this?
Kilaza Samson is offline   Reply With Quote

biom, megan5, mothur meta-analysis

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