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Old 03-19-2016, 04:26 PM   #1
Min Jung Lee
Location: Seoul Korea

Join Date: Jan 2014
Posts: 11
Default Please help me Miseq stopped run with error.

Hello, everyone.
I ran miseq and 3hours later had been stopped.
"Focus mirror sensor not found" error message.
But! Thumnail images are present 2 cycles.
Clusters is good by the thumnail images.
Error message is below.
"Focus mirror move sensor and/or sensor failed:
The FPGA reported and error while exacuting command "FOCMRRFOC"
error message."
Anyone had experience about this error? And what happend to next?

Last edited by Min Jung Lee; 03-19-2016 at 05:29 PM.
Min Jung Lee is offline   Reply With Quote
Old 03-19-2016, 04:58 PM   #2
Senior Member
Location: East Coast USA

Join Date: Feb 2008
Posts: 7,030

This is definitely a hardware related error that none of us can help you with. Please contact Illumina tech support. Include a screenshot of the error in your ticket.
GenoMax is offline   Reply With Quote
Old 03-22-2016, 05:08 AM   #3
Junior Member
Location: Antwerp, Belgium

Join Date: Mar 2015
Posts: 4

Contact tech support just to be sure.

Probably an hardware issue. But make sure the instrument is not upgraded to MCS . We used to have similar issues (but can't remember the exact message) a couple of months ago. Loss of focus occured during the run.

Since our instruments are downgraded to the previous version 2.5.x.x everything seems to work fine again.
Tim NGS is offline   Reply With Quote
Old 03-24-2016, 11:09 PM   #4
Min Jung Lee
Location: Seoul Korea

Join Date: Jan 2014
Posts: 11
Default Problem solved.

I supported illumina tech team.
But error message is poped up again.
So, I contact illumina engineer in Korea.
They checked our instrument about focus mirror part.
And they diagnosed focusing mirror's movements are vey bad, replcement mirror and motor.
Finally, I ran again same libray.
Results is very good Q30 is 94.5% and PF is 93.5%!!
Thank you for everyone.
Min Jung Lee is offline   Reply With Quote

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