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Old 07-08-2016, 11:25 AM   #1
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Location: New Haven

Join Date: Mar 2016
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Unhappy Disproportionate Ratio of Index Adapters

So my protocol is using a custom P5 index adapter with a standard Illumina Nextera N70X oligo. I made the mistake of using a P5 aliquot that is somewhere between 10 and 100x more concentrated than it should have been.

Aside from primer dimer formation which I can fix, are there any other potential issues this may have caused?

I need to decide whether I should toss these libraries and run tagmentation on a new batch, or send this batch to sequencing after PCR purification.
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Old 07-12-2016, 07:00 AM   #2
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Location: Washington, D.C. metro area

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I think you might be okay? If possible, a bioanalyzer run on the completed libraries could help you figure out if there are improperly formed products and if the size of the main insert/library is correct. I'd say that if everything looks as expected, you should be okay to proceed to sequencing?
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