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Old 10-22-2013, 07:17 AM   #1
Zaag
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Default SOLiD5500 (total) meltdown

Hello,

we are experiencing problems with our 2 SOLiD5500 machines since end of july. It is getting extremely frustrating because one run the reverse quality is crap (<40% good best), another one the forward is crap (<20% good best)and the next one the barcode is crap (<60% good best).

Is there anyone else experiencing problems with their machines when comparing results to previous ones?

Sidenote: we do see that lane 1 is routinely better than lane 6, although lifetech assured us this was impossible.
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Old 10-25-2013, 08:36 PM   #2
snetmcom
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Are you certain the library titration was correct? Best beads % can be low from too much DNA in bead prep.
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Old 10-30-2013, 02:42 AM   #3
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Yes I am sure the samples are good, we did run them previously without problems. The noise/signal and on axis are all ok.

The problem does not appear to be the machine so now we are testing another EZbead station and another lot-nr chemistry.
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Old 11-06-2013, 01:59 AM   #4
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I give up.

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Old 11-07-2013, 07:03 PM   #5
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that does not look like solid data. shouldn't there be cycles of quality?
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Old 11-07-2013, 11:46 PM   #6
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You mean like this?



Last bar of the first ligation cycle is base 71
Last bar of the second ligation cycle is base 72

etc.
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