Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Amount of starting material for DNA library prep jport Sample Prep / Library Generation 2 09-24-2014 12:12 AM
sequalprep normalization of amplicons for titanium Seqasaurus Sample Prep / Library Generation 4 03-12-2014 03:16 AM
How much starting material? hambone 454 Pyrosequencing 1 01-20-2012 05:38 AM
amount of starting material for a 454 run hambone Sample Prep / Library Generation 0 01-04-2012 03:36 PM
concentration of my starting material? seqgirl123 Illumina/Solexa 6 07-28-2009 06:19 PM

Thread Tools
Old 03-15-2016, 05:19 AM   #1
Junior Member
Location: Finland

Join Date: Sep 2013
Posts: 1
Default SequalPrep normalization plates and low amount of starting material


I have been looking into Invitrogen's (ThermoFisher Scientific) SequalPrep normalization plates for a quick way to clean and normalize barcoded DNA-libraries (amplicons) for IonTorrent sequencing. The protocol looks easy and the price is not too bad, so I'd love to use this method instead of our current individual Qubit measurements and pooling which works fine but is quite time consuming.

My main concern is with the amount of starting material that is required in the protocol, "at least 250 ng PCR product (amplicon)". I don't think I have this much DNA after my PCR which is low in volume and has a lowish number of cycles. Has anyone else used the SequalPrep kit with lower DNA amounts than specified in the protocol? Would it still work if starting with less than 250 ng??

meri.lindqvist is offline   Reply With Quote

amplicon, normalization, pooling pcr, sequalprep

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 03:14 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO