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Old 06-21-2011, 02:03 PM   #1
floraliu
Junior Member
 
Location: california

Join Date: May 2011
Posts: 1
Post Hi, one question about RNA-Seq library prep

Hi all,

I'm new in this field and want to learn something here.

I'm doing RNA-Seq library prep, we need to get good libraries from small starting total RNA material. When i tried to use 500ng total RNA to start, i usually couldn't get more than 1ng of purified cDNA. Does anyone have some suggestions on how to get more cDNA?


Thanks
-Flora
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