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Old 07-12-2011, 06:15 PM   #1
hawainpanda
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Default repeating chipseq or chipseq with another antibody

I was wondering which was the preferred method?
Would people prefer performing chipseq with two diff antibodies, or repeating chipseq with same antibody?
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Old 03-12-2015, 03:34 AM   #2
mziemann
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Not sure what you mean. Once an antibody is validated as specific, it should be fine to use. The more biological and technical replicates you perform the better.
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Old 03-12-2015, 03:56 PM   #3
kerplunk412
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As a reviewer, ChIP-Seq with a different antibody would definitely add strength to a manuscript. Biological triplicates with both antibodies would be ideal. I think people sometimes put too much faith in antibody specificity, so I think data generated with two different antibodies is much more convincing. Also, an IP-Western with an IP done with one antibody and the Western done with another is always a strong piece of data to add to a manuscript, in my opinion.
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