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Old 05-20-2015, 11:28 AM   #1
linda2015
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Post barcode library for ion torrent

Hi everybody, I am new in the NGS field. I want to ask a question. If I want to barcode my samples, should I do paired barcode or one side barcode, considering the reading length of ion torrent, If the fragment is more than 300bp, maybe there will be an issue to do paired barcod?

I want to do a pilot experiment to test my propose. but I hardly find literature regarding to my question.

I wonder if any of you have experience on this, can you share your thoughts?

Greatly appreciated!
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Old 05-20-2015, 09:52 PM   #2
maven
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One barcode because Ion Torrent software doesn't look for barcodes on the other ends.
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Old 05-21-2015, 08:43 AM   #3
linda2015
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Quote:
Originally Posted by maven View Post
One barcode because Ion Torrent software doesn't look for barcodes on the other ends.
thanks for your reply,
Another question is, I am not sure in which end to put barcode, in the beads end, is it P1 adapter and the other is A adpater? If I want to add barcode for sucessful read through, which end is better.

Thanks a lot
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