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Old 03-21-2016, 04:02 AM   #1
ricardoalves85
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Location: Lisbon

Join Date: Mar 2016
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Post SOLiD number of reads

Dear all,

In the same sequencing run, we sequenced three different biological replicates from the same development stages (n = 3).

However, different raw reads were obtained in one of these libraries (e.g. LIBA - 100,000,000; LIBB 6,500,00 and LIB3 6,300,00).

Should be this high read number cDNA library excluded from the differential expressed analyis? And What can be occured during library production and sequencing to obtain this strange result? As at the same time we sequenced another 12 libraries (other stages), and the number o read was around 6,000,000.

regards,
ricardo
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