I was aligning short reads obtained from Illumina but always got an error as following:
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perl bswc_bowtie2_align.pl --out=./outReadslevel/ --sam=./outputSAM/chr ./h.sapiens.bsIndex/chr ./h.sapiens.ref/chr*.fa ./SRA/sra_data.fastq
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Error: <mate1s/reads> not specified
The alignment pipeline is followed by:
# sra_data.fastq is built successfully in the first step..
Does anyone know how to solve this problem?
Thank you in advance!!
###############################################
perl bswc_bowtie2_align.pl --out=./outReadslevel/ --sam=./outputSAM/chr ./h.sapiens.bsIndex/chr ./h.sapiens.ref/chr*.fa ./SRA/sra_data.fastq
###############################################
Error: <mate1s/reads> not specified
The alignment pipeline is followed by:
# sra_data.fastq is built successfully in the first step..
Does anyone know how to solve this problem?
Thank you in advance!!
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