Hello,
I am having an issue with my MiSeq and was wondering if any of you have had a similar issue or any ideas of what could be causing the issue.
I went to start a run last friday night and the flow rate failed to acquire for the final check. The error said it could not detect a flow rate. I tried pumping PR2 without any success. And then did a line wash after which the instrument said there may be a clog contact illumina support.
I was advised to try different flow cells, using the wash flow cell the same errors occurred. To do a power cycle and multiple washes (after multiple line washes and maintenance washes there still has been no success). Also, I have done the Volume Test which passed and have done multiple power cycles.
So if the flow cell isn't the issue and If the volume test pass indicates that it isn't a pump, valve, or sensor, then is it certainly a clog? If so how much more washing can be done before we pay for a technician to come out?
One last odd thing is there is an "@@@---failed to get temperature" warning that keeps popping up and has done so through multiple power cycles. Illumina says this wouldn't affect anything and also is not related to the issue, but I thought I would include it since its odd it started happening at the same time.
I'm not sure I understand the issue completely, and I am out of ideas for testing the instrument. If you have any ideas to share it would be much appreciated.
Ryan V
Biomedical Research Centre
Vancouver, BC
I am having an issue with my MiSeq and was wondering if any of you have had a similar issue or any ideas of what could be causing the issue.
I went to start a run last friday night and the flow rate failed to acquire for the final check. The error said it could not detect a flow rate. I tried pumping PR2 without any success. And then did a line wash after which the instrument said there may be a clog contact illumina support.
I was advised to try different flow cells, using the wash flow cell the same errors occurred. To do a power cycle and multiple washes (after multiple line washes and maintenance washes there still has been no success). Also, I have done the Volume Test which passed and have done multiple power cycles.
So if the flow cell isn't the issue and If the volume test pass indicates that it isn't a pump, valve, or sensor, then is it certainly a clog? If so how much more washing can be done before we pay for a technician to come out?
One last odd thing is there is an "@@@---failed to get temperature" warning that keeps popping up and has done so through multiple power cycles. Illumina says this wouldn't affect anything and also is not related to the issue, but I thought I would include it since its odd it started happening at the same time.
I'm not sure I understand the issue completely, and I am out of ideas for testing the instrument. If you have any ideas to share it would be much appreciated.
Ryan V
Biomedical Research Centre
Vancouver, BC
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