Dear NGS community.
Please can you give advice on the following:
I have 24Million paired end 50bp reads from human GII transcriptomic sequencing. I would like to know the following:
1) Assemble reads into full length transcripts
2) Identify which transcripts are novel (ie, without having to manually view the whole human genome in a browser).
I have read your pages on software and see programs like velvet and tophat that could do step 1. Does anyone have experience of doing this type of analysis and know a basic pipeline I could follow?
Thanks in advance of any advice.
Kind regards,
John.
Please can you give advice on the following:
I have 24Million paired end 50bp reads from human GII transcriptomic sequencing. I would like to know the following:
1) Assemble reads into full length transcripts
2) Identify which transcripts are novel (ie, without having to manually view the whole human genome in a browser).
I have read your pages on software and see programs like velvet and tophat that could do step 1. Does anyone have experience of doing this type of analysis and know a basic pipeline I could follow?
Thanks in advance of any advice.
Kind regards,
John.
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