Hey I am using the agi exome capture kit. My capture is done and now I would like to confirm if my capture worked. What is the best validation method to do so?
I thought of using a qPCR with primers against 3 exons and 3 introns and compare the dna samples before and after capture. The ideal result should be that the exons are enriched in samples after capture as against introns. But the issue is since I dont have a control I dont know how to calculate a delta delta ct. I am missing a loading control.
Any suggestions?
Thanks
Arvi
I thought of using a qPCR with primers against 3 exons and 3 introns and compare the dna samples before and after capture. The ideal result should be that the exons are enriched in samples after capture as against introns. But the issue is since I dont have a control I dont know how to calculate a delta delta ct. I am missing a loading control.
Any suggestions?
Thanks
Arvi
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