Hi everyboy. I have a quick question about the "--library-type" option in the Tophat2. If the reads are from single end, RNA-seq (poly(A)) LIbrary using kit Ion Total RNA-Seq Kit v2 which options is correct. FR Unstranded? the another are -FR- First strand or FR-Second Strand. Can Anyone clarify me this?
Thanks in advance
Regards
Thanks in advance
Regards
Comment