Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • #1

    Strand information for alignment of KAPA mRNA HyperPrep Kit data

    Hello,
    I am looking on data generated with KAPA mRNA HyperPrep Kit. this library prep is stranded. When I run Salmon/Histat2 for alignment, it asks whether the data is strandedF/strandedR/unstranded. I ran all options and they give slightly different results.
    Is the option I should use the Stranded F? Also, if anyone can direct me to an explanation on how you get stranded information that would be helpful.

    Thank you!!!
    Tags: None
Previous template Next

Latest Articles

Collapse
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM
View All

ad_right_rmr

Collapse

News

Collapse
Topics Statistics Last Post
Cancer Metastasis: A Deep Dive into Cellular Plasticity by seqadmin
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
18 views
0 likes
 Last Post seqadmin
by seqadmin
04-11-2024, 12:08 PM  
Proteogenomic Profiles Offer New Clues in Prostate Cancer by seqadmin
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
22 views
0 likes
 Last Post seqadmin
by seqadmin
04-10-2024, 10:19 PM  
Novel Diagnostic Assay Enhances Ovarian Cancer Detection by seqadmin
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
16 views
0 likes
 Last Post seqadmin
by seqadmin
04-10-2024, 09:21 AM  
Evolutionary Dynamics of Centromeres: A Comparative Genomic Analysis by seqadmin
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
46 views
0 likes
 Last Post seqadmin
by seqadmin
04-04-2024, 09:00 AM  
View All
Working...
X