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Old 08-13-2016, 01:53 PM   #1
pheucticus
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Location: NYC

Join Date: May 2016
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Default SMART-Seq v4 Ultra Low Input RNA Kit potentially compromised - how robust are the rea

Hello,

Sadly there was a freezer malfunction last weekend, and one of the items that may have been compromised was a brand new SMART-Seq v4 Ultra Low Input RNA Kit. Our best estimate is that the kit was left at ~4C for three days. How robust are the oligos, buffers, and enzymes (SeqAmp DNA Polymerase and SMARTScribe Reverse Transcriptase) to being left at this temperature for this length of time? I am most concerned about the enzymes, though I am unsure about the buffers and oligos.

Thanks very much for any insight you can provide.
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