Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Correct Ratio for two-sided PCR-cleanup with Ampure XP beads

    Hey guys,
    I want to use Ampure XP beads for size selection after PCR. I tried to find the right ratio of beads : pcr-product but got mixed results so far.

    I'm curious, what ratios should work approximately for a two-sided clean-up if i want to select pcr-products around 170-230bp.

    I would like to have a very clean product. The PCR is run with genomic DNA which has been sheared to a length of around 10000bp and the Primers used are around 55bp. Both should be cleaned out as well as possible.

    Thanks for the input.

  • #2
    Double SPRI size selection window will be much larger than 50 bp. For your target you need to use Sage Pippin or gel extraction.

    Comment


    • #3
      Take a look at https://ls.beckmancoulter.co.jp/file...SPRIselect.pdf
      But I agree with nucacidhunter that 50 bp is a too narrow window for a double-SPRI

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM
      • seqadmin
        Techniques and Challenges in Conservation Genomics
        by seqadmin



        The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

        Avian Conservation
        Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
        03-08-2024, 10:41 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Yesterday, 06:37 PM
      0 responses
      10 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, Yesterday, 06:07 PM
      0 responses
      9 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-22-2024, 10:03 AM
      0 responses
      49 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-21-2024, 07:32 AM
      0 responses
      67 views
      0 likes
      Last Post seqadmin  
      Working...
      X