Hello everyone,
I'm thinking about trying my hand at assembling some ~ 300 MBp genomes using 100 bp paired-end Illumina reads (coverage is not huge ~ 8-12X). I'm not new to bioinformatics, but new to this area generally. Does anyone have any preferences for which assemblers work best for this kind of data? I'm not sure if I should attempt to use a ref genome for a guide since the most closely related species is not that closely related.
Any pointers much appreciated.
Thanks,
Michael
I'm thinking about trying my hand at assembling some ~ 300 MBp genomes using 100 bp paired-end Illumina reads (coverage is not huge ~ 8-12X). I'm not new to bioinformatics, but new to this area generally. Does anyone have any preferences for which assemblers work best for this kind of data? I'm not sure if I should attempt to use a ref genome for a guide since the most closely related species is not that closely related.
Any pointers much appreciated.
Thanks,
Michael
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