Hi everyone. I'm working on a small fish genome and want to know if anyone is having any success using the new fast flow cells for HiSeq that give >100bp reads as input along with a 3000bp mate pair read as the input for allpaths rather than making the 180bp library. The fast flow cells generate single reads rather than paired reads which is why I'm not sure if this will work. Any input is appreciated especially if you have tried this.
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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