how to interpret MIRA result

54016565 · 03-23-2012, 08:43 AM

Hi,

I am working on assembly ecoli genome by MIRA with a backbone. Some part of the info report I am not quite understand, if someone can share some of his experience? I am very new in this area, so any explanations would be greatly helpful!

1. most importantly, by default setting, I only get one huge contig which is almost same size as the backbone. My understanding is the assembly process with backbone in MIRA is like mapping reads back, but what about genome location without reads coverage? Besides, For de novo assembly we usually pick the one with more and longer contigs as best assembly, and here how can I evaluate it?

2. My data is from 454, but why "Max coverage per sequencing technology" shows there are some Sanger data

BaCh · 03-26-2012, 03:08 AM

Answer is here: http://www.freelists.org/post/mira_t...-MIRA-result,1

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03-23-2012, 08:43 AM	#1
54016565 Junior Member Location: Washington DC Join Date: Jun 2011 Posts: 5	how to interpret MIRA result Hi, I am working on assembly ecoli genome by MIRA with a backbone. Some part of the info report I am not quite understand, if someone can share some of his experience? I am very new in this area, so any explanations would be greatly helpful! 1. most importantly, by default setting, I only get one huge contig which is almost same size as the backbone. My understanding is the assembly process with backbone in MIRA is like mapping reads back, but what about genome location without reads coverage? Besides, For de novo assembly we usually pick the one with more and longer contigs as best assembly, and here how can I evaluate it? 2. My data is from 454, but why "Max coverage per sequencing technology" shows there are some Sanger data Last edited by 54016565; 03-23-2012 at 02:36 PM.

03-26-2012, 03:08 AM	#2
BaCh Member Location: Germany Join Date: May 2008 Posts: 79	Answer is here: http://www.freelists.org/post/mira_t...-MIRA-result,1