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09-29-2009, 05:16 PM
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#1 |
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Senior Member
Location: China Join Date: Sep 2009
Posts: 199
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Difference between 454 pair end library and 454 standard library
Hi,
Can anybody list out the main difference between the 454 pair end library and 454 standard library? Thanks a lot 
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09-30-2009, 01:08 AM
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#2 |
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Peter (Biopython etc)
Location: Dundee, Scotland, UK Join Date: Jul 2009
Posts: 1,543
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In terms of the SFF files themselves (as far as I know) there is no difference at all. There is nothing in the file format or the Roche XML meta data block that I have seen to tell you if the data is single reads or paired ends.
It is all down to the sample preparation. The Roche 454 website has a nice summary of how this works: http://www.454.com/products-solution...-end-reads.asp If you know you have a paired end SFF file, you should be able to find the linker sequences in the reads - see: http://seqanswers.com/forums/showthread.php?t=2698 |
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