SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > 454 Pyrosequencing
Similar Threads
Thread Thread Starter Forum Replies Last Post
What is the longest fragment for Illumina Mate pair end seq library? ychang Sample Prep / Library Generation 4 03-08-2012 12:11 AM
Illumina Pair end library protocol Berkeley2010 Illumina/Solexa 16 11-18-2010 06:53 AM
SPRI bead for PCR cleanup in illumina pair end library preparation Berkeley2010 Illumina/Solexa 2 10-20-2010 12:46 PM
Difference between mate pair and pair end bassu General 2 06-19-2010 06:13 AM
Fragment Library vs Mate-pair library magicsiew SOLiD 1 02-03-2010 06:50 PM

Reply
 
Thread Tools
Old 09-29-2009, 05:16 PM   #1
edge
Senior Member
 
Location: China

Join Date: Sep 2009
Posts: 199
Default Difference between 454 pair end library and 454 standard library

Hi,
Can anybody list out the main difference between the 454 pair end library and 454 standard library?
Thanks a lot
edge is offline   Reply With Quote
Old 09-30-2009, 01:08 AM   #2
maubp
Peter (Biopython etc)
 
Location: Dundee, Scotland, UK

Join Date: Jul 2009
Posts: 1,543
Default

In terms of the SFF files themselves (as far as I know) there is no difference at all. There is nothing in the file format or the Roche XML meta data block that I have seen to tell you if the data is single reads or paired ends.

It is all down to the sample preparation. The Roche 454 website has a nice summary of how this works:
http://www.454.com/products-solution...-end-reads.asp

If you know you have a paired end SFF file, you should be able to find the linker sequences in the reads - see:
http://seqanswers.com/forums/showthread.php?t=2698
maubp is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off



All times are GMT -8. The time now is 10:07 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2022, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO