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01-04-2014, 04:48 PM
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#1 |
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Member
Location: beijing Join Date: Nov 2011
Posts: 17
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stange kmer content
hi all,
I've got this fastqc report with high ttttt all along the 1st group of paired-end reads, and high aaaaa &&. I TTTTT all along the 2nd group of paired-end reads. is it normal? thanks alot! (nimblegen 70M exome sequencing data, PE reads, 90bp lengh) Last edited by MissDona; 01-04-2014 at 04:55 PM. |
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01-04-2014, 04:58 PM
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#2 |
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Member
Location: beijing Join Date: Nov 2011
Posts: 17
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other images
as attached, thanks!
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01-05-2014, 07:31 AM
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#3 |
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Senior Member
Location: East Coast USA Join Date: Feb 2008
Posts: 7,143
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There is no signal for "C". That is odd. Are all your samples showing this pattern? Were they sequenced on the same flowcell? Have you checked with your sequence provider that there was nothing technically wrong with this run?
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01-08-2014, 07:27 AM
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#4 |
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Junior Member
Location: Oxford, UK Join Date: Mar 2012
Posts: 9
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This is very odd. If you can't trace the cause, consider clipping your reads.
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| fastqc, kmer, ttttt |
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