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Thread | Thread Starter | Forum | Replies | Last Post |
Phred score of i | jmugford | Bioinformatics | 1 | 08-12-2011 05:18 PM |
How to filter reads on PHRED score | sunkorner | RNA Sequencing | 0 | 06-25-2011 03:43 PM |
Phred Score from AB1 file | nxtgenkid10 | General | 0 | 03-08-2011 12:43 AM |
SNP calling - is there an accepted Phred quality threshold? | Francesco Lescai | Bioinformatics | 3 | 04-13-2010 11:51 AM |
Quality score threshold? | chris | Bioinformatics | 8 | 04-29-2008 12:43 AM |
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#1 |
Senior Member
Location: IL Join Date: Jul 2011
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A vcf file processed after those 3 commands below seems has filtered low-phred polymorphic bases. What is the default threshold? Where can I change that threshold in command?
$samtools mpileup -uf Sorbil.fasta sample.bam > sample.pileup $bcftools view -bvcg ample.pileup > var.bcf $bcftools view var.bcf > var.vcf |
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